ISOLATION AND ANTIBIOTICS SUSCEPTIBILITY OF CLOSTRIDIUM PERFRINGENS

Clostridium perfringens is a Gram-positive bacillus that forms spores. Clostridium
perfringens is a common bacteria that cause food poisoning, it is an emerging health problem in health setting. The ability of the spores to persist in the environment is a key factor in rates of infection caused by Clostridium perfringens. Clostridium perfringens has also been described as one of the leading cause of food poisioning. It causes a serious toxic-mediated enteric diseases in humans. This study aimed to investigate the isolation and antibiotic susceptibility of Clostridium perfringens from different samples. A total of forty five (45) samples comprising of sea food (22), wastewater (7), dumpsite soil (16) samples were collected for this study. Isolation was carried out using Reinforced clostridial agar in an anaerobic condition, while identification of this bacteria was done using morphological and biochemical tests. Antibiotic susceptibility was tested in this study using eight (8) antimicrobials disk via disc diffusion method. The isolate gotten from all the samples were eight (8) and all the isolates have the characteristics morphological features of Clostridium
perfringens on Reinforced clostridial agar. The highest antibiotic susceptibility test of the isolates was recorded with Chloramphenicol (87%), tetracycline (75%) , Ciprofloxacin (75%), Ampicillin (62%), Vancomycin (62%), Gentamicin (62%). Erythromycin (25%) and Metronidazole (0). In this study, Clostridium perfringens was isolated more in the food samples than in the environmental samples.